– generating, by means of a suitable sensor included in the detection section, a § transporting the sample injected along the capillary from an injection section to a detection section thereof, in experimental conditions suitable to generate a Taylor dispersion phenomenon that is measurable at the level of the detection section § injecting a sample of the mixture to be analyzed inside an open tubular capillary in which an eluent is flowing This invention concerns methods for determining the size distribution of a mixture of particles or (macro)molecules by implementing the Taylor dispersion and associated system, including the following steps: The invention therefore aims to overcome this problem, in particular by offering a real-time method for analyzing experimental Taylor signal from a sample of any mixture. In practice, it is currently considered impossible to solve the general problem of deconvolution Taylor experimental signal of a sample of any mixture of species. Consequently, these known algorithms do not allow to analyze any sample, but only samples that are previously known to result from the mixing of two kinds. However, these algorithms can be used only in the particular case of a binary mixture, that is to say a mixture of two species.
The international application published under number WO 2010 009907 A1 discloses a method of the aforementioned type which the analysis step implements various deconvolution algorithms an experimental Taylor dispersion signal. The term "deconvolution" of a Taylor signal consisted in finding the hydrodynamic radius of each component of the mixture species and determining the concentration of each of these species. Taylor Dispersion Analysis is a simple analytical technique leading to the size characterization of a sample mixture via the analysis of the dispersion of a sample band in an open capillary tube.
0 kommentar(er)
